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image-it® lipid peroxidation sensor c10445  (Thermo Fisher)


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    Thermo Fisher image-it® lipid peroxidation sensor c10445
    Image It® Lipid Peroxidation Sensor C10445, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lipid+peroxidation+sensor/image+it+lipid+peroxidation+kit/pm40494997-343-8-12
    Average 90 stars, based on 1 article reviews
    image-it® lipid peroxidation sensor c10445 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Identification of A New Kind of Phenazine Compounds Attenuating The Stemness of Breast Cancer Cells Through Triggering Ferroptosis
    Article Snippet: .. 48 h later, the cells were stained with 10 μM Lipid peroxidation sensor (ThermoFisher, Cat# B3932) for 30 min at 37°C. ..

    Article Title: Heme: a link between hemorrhage and retinopathy of prematurity progression.
    Article Snippet: .. Cells were 794 stained with Hoechst 33342 and 10 μM of Lipid Peroxidation Sensor (Thermo Fisher Scientific, 795 Waltham, MA, US) for 30 mins incubated at 37°C. .. Cells were then washed three times with 796 phosphate buffered saline and imaged in Hank’s Balanced Salt Solution with calcium and 797 magnesium with Leica confocal microscope using filters for Hoechst, FITC and Texas Red 798 channels.

    Article Title: Phenazine derivatives attenuate the stemness of breast cancer cells through triggering ferroptosis
    Article Snippet: .. 48 h later, cells were stained with 10 μM Lipid peroxidation sensor (ThermoFisher, Cat# B3932) for 30 min at 37°C. ..

    Article Title: Heme: A link between hemorrhage and retinopathy of prematurity progression
    Article Snippet: .. Cells were stained with Hoechst 33342 and 10 μM of Lipid Peroxidation Sensor (Thermo Fisher Scientific, Waltham, MA, US) for 30 min incubated at 37 °C. .. Cells were then washed three times with phosphate buffered saline and imaged in Hank's Balanced Salt Solution with calcium and magnesium with Leica confocal microscope using filters for Hoechst, FITC and Texas Red channels.

    Incubation:

    Article Title: Heme: a link between hemorrhage and retinopathy of prematurity progression.
    Article Snippet: .. Cells were 794 stained with Hoechst 33342 and 10 μM of Lipid Peroxidation Sensor (Thermo Fisher Scientific, 795 Waltham, MA, US) for 30 mins incubated at 37°C. .. Cells were then washed three times with 796 phosphate buffered saline and imaged in Hank’s Balanced Salt Solution with calcium and 797 magnesium with Leica confocal microscope using filters for Hoechst, FITC and Texas Red 798 channels.

    Article Title: Heme: A link between hemorrhage and retinopathy of prematurity progression
    Article Snippet: .. Cells were stained with Hoechst 33342 and 10 μM of Lipid Peroxidation Sensor (Thermo Fisher Scientific, Waltham, MA, US) for 30 min incubated at 37 °C. .. Cells were then washed three times with phosphate buffered saline and imaged in Hank's Balanced Salt Solution with calcium and magnesium with Leica confocal microscope using filters for Hoechst, FITC and Texas Red channels.

    Modification:

    Article Title: Effect of Low Concentration of Nitroxides on SH-SY5Y Cells Transfected with the Tau Protein
    Article Snippet: JC-1 Mitochondrial Membrane Potential Assay Kit (cat. no. ab113850) was obtained from Abcam (Cambridge, UK). .. Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 without phenol red (cat. no. 21041025), Dulbecco’s phosphate-buffered saline (DPBS; cat. no. 14040-117), and Lipid Peroxidation Sensor [(4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-un-decanoic,] (C11-BODIPY ® 581/591; CAS no. 217075-36-0, cat. no. D3861) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Fetal bovine serum (cat. no. S1813), penicillin–streptomycin solution (cat. no. L0022), Trypsin–EDTA solution (10×) (cat. no. X0930), and phosphate-buffered saline without Ca 2+ and Mg 2+ (cat. no. P0750) were obtained from Biowest (Nuaillé, France).

    Saline:

    Article Title: Effect of Low Concentration of Nitroxides on SH-SY5Y Cells Transfected with the Tau Protein
    Article Snippet: JC-1 Mitochondrial Membrane Potential Assay Kit (cat. no. ab113850) was obtained from Abcam (Cambridge, UK). .. Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 without phenol red (cat. no. 21041025), Dulbecco’s phosphate-buffered saline (DPBS; cat. no. 14040-117), and Lipid Peroxidation Sensor [(4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-un-decanoic,] (C11-BODIPY ® 581/591; CAS no. 217075-36-0, cat. no. D3861) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). .. Fetal bovine serum (cat. no. S1813), penicillin–streptomycin solution (cat. no. L0022), Trypsin–EDTA solution (10×) (cat. no. X0930), and phosphate-buffered saline without Ca 2+ and Mg 2+ (cat. no. P0750) were obtained from Biowest (Nuaillé, France).



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    bodipytm 581/591 c11 (lipid peroxidation sensor)  (Thermo Fisher)
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    Fluorescence images taken at a nominal magnification of 20x of (A) A549 and (B) BEAS-2B cells treated with RSL3 (A549: 10 µM and BEAS-2B: 250 nM) or infected with H1N1/PR8 influenza virus at MOI 1 stained with <t>BODIPY</t> TM 581/591 <t>C11</t> at 24 hpi and counterstained with Hoechst 33258. Red: non-oxidized lipids (C11 Non-Ox ); Green: oxidized lipids (C11 Ox ) Blue: nucleus (scale bar = 100 µm). Quantitative analysis of mean integrated fluorescence intensity of C11 Ox /C11 Non- Ox ratio was measured for both (C) A549 and BEAS-2B cells (mean ± SD). Each dot represents the quantification of one biological replicate. At least 10 images per condition per biological replicate were analyzed. C11 Ox /C11 Non-Ox ratio of control was defined as 100%. (D) Schematic representation of iron participation in Fenton reaction leading to oxidation of polyunsaturated fatty acids (PUFAs), the production of malondialdehyde (MDA), and representation of iron transport into the cell by transferrin receptor (Tf), as well as the subsequent storage of intracellular iron in ferritin. (E) MDA levels of A549 and BEAS-2B cell lysates collected at 24 hpi with influenza H1N1/PR8 and treatment with RSL3 (A549:10 µM and BEAS-2B: 250 nM) and pre-treatment with Fer-1 (5 µM). MDA concentrations were normalized to the corresponding protein concentration and are presented as fold changes to the control. (F) Representative blot showing the relative expression of FTL in A549 and BEAS-2B cells infected with influenza H1N1/PR8 at a MOI = 1 or treated with RSL3 (A549: 30 µM and BEAS-2B: 300 nM). Cell lysates were harvested at 24 hpi for Western blot analysis. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test. *p < 0.05, **p < 0.01 ***p < 0.001. Data are represented as mean ± SD of at least three independent biological replicates.
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    Image Search Results


    Fluorescence images taken at a nominal magnification of 20x of (A) A549 and (B) BEAS-2B cells treated with RSL3 (A549: 10 µM and BEAS-2B: 250 nM) or infected with H1N1/PR8 influenza virus at MOI 1 stained with BODIPY TM 581/591 C11 at 24 hpi and counterstained with Hoechst 33258. Red: non-oxidized lipids (C11 Non-Ox ); Green: oxidized lipids (C11 Ox ) Blue: nucleus (scale bar = 100 µm). Quantitative analysis of mean integrated fluorescence intensity of C11 Ox /C11 Non- Ox ratio was measured for both (C) A549 and BEAS-2B cells (mean ± SD). Each dot represents the quantification of one biological replicate. At least 10 images per condition per biological replicate were analyzed. C11 Ox /C11 Non-Ox ratio of control was defined as 100%. (D) Schematic representation of iron participation in Fenton reaction leading to oxidation of polyunsaturated fatty acids (PUFAs), the production of malondialdehyde (MDA), and representation of iron transport into the cell by transferrin receptor (Tf), as well as the subsequent storage of intracellular iron in ferritin. (E) MDA levels of A549 and BEAS-2B cell lysates collected at 24 hpi with influenza H1N1/PR8 and treatment with RSL3 (A549:10 µM and BEAS-2B: 250 nM) and pre-treatment with Fer-1 (5 µM). MDA concentrations were normalized to the corresponding protein concentration and are presented as fold changes to the control. (F) Representative blot showing the relative expression of FTL in A549 and BEAS-2B cells infected with influenza H1N1/PR8 at a MOI = 1 or treated with RSL3 (A549: 30 µM and BEAS-2B: 300 nM). Cell lysates were harvested at 24 hpi for Western blot analysis. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test. *p < 0.05, **p < 0.01 ***p < 0.001. Data are represented as mean ± SD of at least three independent biological replicates.

    Journal: bioRxiv

    Article Title: Respiratory viruses induce ferroptosis-like features in lung epithelial cells

    doi: 10.1101/2025.10.29.685341

    Figure Lengend Snippet: Fluorescence images taken at a nominal magnification of 20x of (A) A549 and (B) BEAS-2B cells treated with RSL3 (A549: 10 µM and BEAS-2B: 250 nM) or infected with H1N1/PR8 influenza virus at MOI 1 stained with BODIPY TM 581/591 C11 at 24 hpi and counterstained with Hoechst 33258. Red: non-oxidized lipids (C11 Non-Ox ); Green: oxidized lipids (C11 Ox ) Blue: nucleus (scale bar = 100 µm). Quantitative analysis of mean integrated fluorescence intensity of C11 Ox /C11 Non- Ox ratio was measured for both (C) A549 and BEAS-2B cells (mean ± SD). Each dot represents the quantification of one biological replicate. At least 10 images per condition per biological replicate were analyzed. C11 Ox /C11 Non-Ox ratio of control was defined as 100%. (D) Schematic representation of iron participation in Fenton reaction leading to oxidation of polyunsaturated fatty acids (PUFAs), the production of malondialdehyde (MDA), and representation of iron transport into the cell by transferrin receptor (Tf), as well as the subsequent storage of intracellular iron in ferritin. (E) MDA levels of A549 and BEAS-2B cell lysates collected at 24 hpi with influenza H1N1/PR8 and treatment with RSL3 (A549:10 µM and BEAS-2B: 250 nM) and pre-treatment with Fer-1 (5 µM). MDA concentrations were normalized to the corresponding protein concentration and are presented as fold changes to the control. (F) Representative blot showing the relative expression of FTL in A549 and BEAS-2B cells infected with influenza H1N1/PR8 at a MOI = 1 or treated with RSL3 (A549: 30 µM and BEAS-2B: 300 nM). Cell lysates were harvested at 24 hpi for Western blot analysis. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test. *p < 0.05, **p < 0.01 ***p < 0.001. Data are represented as mean ± SD of at least three independent biological replicates.

    Article Snippet: Lipid peroxidation was determined using BODIPY 581/591 C11 Lipid Peroxidation Sensor ((#D3861, Fisher Scientific, Landsmeer, the Netherlands) [ , , ].

    Techniques: Fluorescence, Infection, Virus, Staining, Control, Protein Concentration, Expressing, Western Blot

    Representative fluorescence images taken at a nominal magnification of 20x of (A) A549 and (B) BEAS-2B cells treated with RSL3 (A549:10 µM and BEAS-2B: 250 nM) for 24 h or infected with HAdV-C5 at a MOI = 1 stained with BODIPY TM 581/591 C11 at 72 hpi and counterstained with Hoechst 33258. Red: non-oxidized lipids (C11 Non-Ox ); Green: oxidized lipids (C11 Ox ) Blue: nucleus (scale bar = 100 µm). (C) Quantitative analysis of mean integrated fluorescence intensity of C11 Ox /C11 Non-Ox ratio was measured for both A549 and BEAS-2B cells (mean ± SD) at 24, 48 and 72 hpi. Each dot represents the quantification of one biological replicate. At least 10 images per condition per biological replicate were analyzed. C11 Ox /C11 Non-Ox ratio of control was defined as 100%. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s comparisons test. (D) MDA levels of A549 cell lysates collected at 24, 48 and 72 hpi with HAdV-C5 and treatment with RSL3 (10 µM) and pre-treatment with Fer-1 (5 µM). MDA concentration was normalized to the corresponding protein concentration and are presented as fold changes compared to the control. Data are represented as mean ± SD of at least three independent biological experiments with three technical replicates. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc multiple comparisons test. *p < 0.05, **p < 0.01.

    Journal: bioRxiv

    Article Title: Respiratory viruses induce ferroptosis-like features in lung epithelial cells

    doi: 10.1101/2025.10.29.685341

    Figure Lengend Snippet: Representative fluorescence images taken at a nominal magnification of 20x of (A) A549 and (B) BEAS-2B cells treated with RSL3 (A549:10 µM and BEAS-2B: 250 nM) for 24 h or infected with HAdV-C5 at a MOI = 1 stained with BODIPY TM 581/591 C11 at 72 hpi and counterstained with Hoechst 33258. Red: non-oxidized lipids (C11 Non-Ox ); Green: oxidized lipids (C11 Ox ) Blue: nucleus (scale bar = 100 µm). (C) Quantitative analysis of mean integrated fluorescence intensity of C11 Ox /C11 Non-Ox ratio was measured for both A549 and BEAS-2B cells (mean ± SD) at 24, 48 and 72 hpi. Each dot represents the quantification of one biological replicate. At least 10 images per condition per biological replicate were analyzed. C11 Ox /C11 Non-Ox ratio of control was defined as 100%. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s comparisons test. (D) MDA levels of A549 cell lysates collected at 24, 48 and 72 hpi with HAdV-C5 and treatment with RSL3 (10 µM) and pre-treatment with Fer-1 (5 µM). MDA concentration was normalized to the corresponding protein concentration and are presented as fold changes compared to the control. Data are represented as mean ± SD of at least three independent biological experiments with three technical replicates. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc multiple comparisons test. *p < 0.05, **p < 0.01.

    Article Snippet: Lipid peroxidation was determined using BODIPY 581/591 C11 Lipid Peroxidation Sensor ((#D3861, Fisher Scientific, Landsmeer, the Netherlands) [ , , ].

    Techniques: Fluorescence, Infection, Staining, Control, Concentration Assay, Protein Concentration